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The cells had been constantly cultured in comprehensive medium for 48 h, and after that the Beckman Coulter MoFlo Astrios Mobile Sorter was utilised to pick out cells with CXCR4-adverse expression. These chosen cells were being cultured in full medium supplemented with 1 μg/mL of puromycin.Ollie123 stated: Undoubtedly haven't and i am sorry you